Fig. 1. Schematic diagram representing the experimental design of HL-60 cells (n= 5) pre-incubated for 24 hours in either cell culture media +/- Sal (200 μM), Nig (300 μM) or a combination of Sal (100 μM), Nig (150 μM) [Arm1]. Cell were cryopreserved in DMSO alone [Arm 2], DMSO + Nig [Arm 2], DMSO +Sal [Arm 3] or in DMSO + Nig and Sal [Arm 4]. Biochemical assays (LDH, lipid peroxidation and ROS) were performed prior and 1 h post thaw and LC-MS lipidomic analysis was carried out 24h post thaw.